Rag2 Knockout Rat
Model Detail >
- Cancer metastasis
- Vaccine development
- Inflammation/Autoimmune disorders
- Thrombosis/Cardiac fibrosis
- Vascular defects
- Infectious disease
DownloadsFile size: 26.28k
Rag2 knockout rats completely lack B and T cells, making them ideal immuno-compromised hosts for xenograft studies. In addition, the larger size of the rat makes performing sophisticated xenograft techniques and surgeries much simpler as compared to similar studies in a mouse.
Request a quote and a SAGE® Labs research specialist will contact you with an estimated price and delivery date.
- 2 bp deletion within Exon 3 on chromosome 3
- Homozygous Rag2 knockout rats display loss of RAG2 protein via Western blot
- Homozygous Rag2 knockout rats show loss of B and T cells by FACS analysis
Figure 1. Flow cytometric analysis of T cell subsets (CD4 and CD8), B cells and NK cells from wild type and Rag2 (-/-) knockout blood samples
The top panels represent wild type female and male rats. The lower panels represent samples from female and male Rag2 (-/-) knockout rats. Blood samples were collected from 4- and 10-week-old rats.
Mature B and T cells are critical components for an adaptive immune system. Rats deficient in Rag2 protein produce no mature B or T cells. This non-leaky model for severe combined immune deficiency is useful for vaccine development, autoimmune and infectious diseases.