Rag1 Knockout Rat
Model Detail >
- Cancer metastasis
- Vaccine development
- Inflammation/Autoimmune disorders
- Thrombosis/Cardiac fibrosis
- Vascular defects
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Recombination Activation Genes (Rag) encode enzymes that play an important role in the rearrangement and recombination of the genes of immunoglobulin and T cell receptor molecules during the process of V(D)J recombination. Rag1 knockout rats lack mature B and T lymphocytes.
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- 29 bp deletion within Exon 2 on chromosome 3
- Homozygous Rag1 knockout rats display loss of RAG1 protein via Western blot
- Homozygous Rag1 knockout rats show loss of B and T cells by FACS analysis
- Background Strain: Sprague-Dawley
Figure 1. Flow cytometric analysis of T cell subsets (CD4 and CD8), B cells and NK cells from wild type and Rag1 (-/-) knockout blood samples
The top panels represent wild type female and male rats. The lower panels represent samples from female and male Rag1 (-/-) knockout rats. Blood samples were collected from 4-, 10- and 20-week-old rats.
Mature B and T cells are critical components for an adaptive immune system. Rats deficient in Rag1 protein produce no mature B or T cells. This non-leaky model for severe combined immune deficiency is useful for vaccine development, as well as the study of autoimmune and infectious diseases.